Table 1.
In vitro embryonic development of embryos collected from d-1 pregnant Dicer1fl/fl;Amhr2Cre/+ and wild-type females
| Day of embryo culture
|
|||||
|---|---|---|---|---|---|
| 2 | 3 | 4 | 5 | ||
| Stage of embryo development | ≥2 cells | ≥4 cells | ≥ Compacting morula | ≥ Blastocyst | ≥ Hatching blastocyst |
| Genotype of donor female (n = embryos; dams) | |||||
| Wild-type (n = 75; 9) | 100% | 96% | 90.7% | 86.7% | 54.7% |
| Dicer1fl/fl;Amhr2Cre/+ (n = 37; 7) | 97% | 86% | 91.7% | 72.2% | 38.9% |
Percentage of two or more cell embryos was calculated from total numbers of fertilized oocytes (n = 75 and 37) for each genotype. Percentages of four or more cell embryos and all subsequent groups were calculated from the number of two or more cell embryos present on d 2 culture.