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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Neurochem Int. 2008 Jun 12;53(3-4):79–88. doi: 10.1016/j.neuint.2008.06.003

Figure 2. A-705253 did not induce changes in dynamin 1 and tau levels in cultured hippocampal neurons.

Figure 2

(A) Western blot analysis of whole cell extracts prepared from 3 weeks in culture hippocampal neurons cultured in the absence (c) or presence of increasing concentrations of A-705253 (inhibitor) and reacted with dynamin 1 (dyn 1) and tau antibodies. No cleaved dynamin 1 or 17 kDa tau bands were detected in treated neurons. Tubulin was used as a loading control. (B & C) Graphs showing the levels of full-length dynamin 1(~100 kDa) and tau (17 kDa/full-length ratio) in control and A-705253-treated hippocampal neurons. Values are expressed as percentage of untreated controls, considering the values obtained in these neurons as 100%. Each number represents the mean ± SEM from 3 different experiments.