Figure 2. IFNAR expression by DC alters the balance between the Th17 and Th1 response.
2D2 T cells were incubated with BM-derived DC and 1 μg/ml of OT-2 peptide in cultures skewed to Th17 [rIL-6 and rTGFβ (A)] or Th1 [rIL-12 and IL-4 Ab (B)]. Supernatants harvested 24h after re-stimulation with soluble CD3 Ab were analyzed by ELISA in triplicate wells shown as mean ± SEM; representative of 5 and 3 experiments for (A) and (B), respectively.