Figure 5. Confocal microscopy of UL97-dependent nuclear lamina alterations in HCMV-infected cells.
At 96 hours p.i. (MOI = 1), cells were fixed and double stained for UL44 (red) and lamin A/C (green) and imaged by confocal microscopy. Discontinuities in lamin A/C staining were detected in wt AD169-infected cells (A) and (B) and are labeled with white arrows. Lamin A/C staining was more uniform and intense around the nuclear rim and gaps in staining were rarely observed in cells infected with AD169 in the presence of maribavir (MBV) (C), when a UL97 deletion mutant virus was used (Δ97) (D), or in mock-infected cells (E).