Fig. 1.
Anthrax ETx inhibits Nox1 activity in HT-29 cells. a HT-29 cells were treated with or without 1 µg/ml PA, 0.5 µg/ml EF, or ETx (1 µg/ml PA plus 0.5 µg/ml EF) for 3 h. b Cells were exposed to ETx (1 µg/ml PA plus 0.5 µg/ml EF) for the indicated time periods. c Cells were treated with various doses of ETx (1 µg/ml PA plus indicated concentration of EF) for 3 h. Nox1 activity was then determined by chemiluminescence assay. Representative experiments from three separate experiments are shown. RLU = Relative light units.