Table 2.
Identification of nitrated sites in subunits from ONOO--treated Complex I with Nano-LC/MS/MS
Spot No. | Subunit Identified | Gene abbreviation | GenInfo Accession No. | Mass (kDa) | Overall Sequence Coverage | Nitrated site identified |
---|---|---|---|---|---|---|
I | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial precursor (NADH-ubiquinone oxidoreductase 24 kDa subunit) | Ndufv2 | 128865 | 27.58 | 70% | none |
II | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial precursor (NADH-ubiquinone oxidoreductase 30 kDa subunit) | Ndufs3 | 128860 | 30.44 | 85% | Y246 |
III | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial precursor (NADH-ubiquinone oxidoreductase 49 kDa subunit) | Ndufs2 | 116242673 | 52.92 | 83% | Y46 and Y52 |
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor (NADH-ubiquinone oxidoreductase 51 kDa subunit) | Ndufv1 | 548387 | 51.30 | 91% | none |
Spot numbers refer to the numbers shown in Fig. 3A.