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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: Cancer Cell. 2008 Oct 7;14(4):312–323. doi: 10.1016/j.ccr.2008.09.001

Figure 2. Deletion of SIRT1 causes chromosome abnormality.

Figure 2

(A) Dapi staining of tissue sections showing abnormal mitotic features (arrows, A-a) in a E10.5 SIRT1−/− embryo. Data (A-b) were collected from 3 pairs of embryos, 200 mitotic phases from each embryo were counted. (B) Chromosome spreads from E9.5 embryos showing normal spread (B-a), aneuploid and abnormal structure, or broken chromosome (arrow, B-b), and less condensed chromosomes (B-c). Chromosome spreads from 9 pairs of embryos were made, and all the spreads from each individual embryo were counted. (C) SIRT1 mutant MEF cells displayed incompletely condensed, and lagging chromosomes (arrow, C-a), and uneven chromosome segregation under a relative normal spindle (α-tubulin staining). Data was summarized in (C-b). Data is presented as average ±SD. Bars: 10 µm for A–B, 20 µm for C.