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. 1983 Nov;42(2):639–644. doi: 10.1128/iai.42.2.639-644.1983

Vibrio cholerae soluble hemagglutinin/protease is a metalloenzyme.

B A Booth, M Boesman-Finkelstein, R A Finkelstein
PMCID: PMC264477  PMID: 6417020

Abstract

A soluble hemagglutinin/protease produced by Vibrio cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions are inhibited by chelating agents, including Zincov, a hydroxamic acid derivative specifically designed to inhibit zinc metalloproteases. Thermolysin, a known zinc-containing protease, also causes hemagglutination of responder chicken erythrocytes. This activity is inhibited by Zincov, which does not affect the hemagglutination activity of trypsin and pronase. The hemagglutinin/protease is active on furylacryloyl-Gly-Leu-NH2, a synthetic substrate for thermolysin and other similar proteases. The hemagglutination activity of V. cholerae-infected or cholera toxin-treated infant rabbit intestinal fluid is not inhibited by Zincov, which suggests that this activity is not due to the hemagglutinin/protease, as formerly proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amphlett G. W., Hrinda M. E. The binding of calcium to human fibronectin. Biochem Biophys Res Commun. 1983 Mar 29;111(3):1045–1053. doi: 10.1016/0006-291x(83)91405-5. [DOI] [PubMed] [Google Scholar]
  2. Feder J., Schuck J. M. Studies on the Bacillus subtilis neutral-protease- and Bacillus thermoproteolyticus thermolysin-catalyzed hydrolysis of dipeptide substrates. Biochemistry. 1970 Jul 7;9(14):2784–2791. doi: 10.1021/bi00816a005. [DOI] [PubMed] [Google Scholar]
  3. Finkelstein R. A., Boesman-Finkelstein M., Holt P. Vibrio cholerae hemagglutinin/lectin/protease hydrolyzes fibronectin and ovomucin: F.M. Burnet revisited. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1092–1095. doi: 10.1073/pnas.80.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finkelstein R. A., Hanne L. F. Purification and characterization of the soluble hemagglutinin (cholera lectin)( produced by Vibrio cholerae. Infect Immun. 1982 Jun;36(3):1199–1208. doi: 10.1128/iai.36.3.1199-1208.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill D. M., Rappaport R. S. Origin of the enzymatically active A1 fragment of cholera toxin. J Infect Dis. 1979 Jun;139(6):674–680. doi: 10.1093/infdis/139.6.674. [DOI] [PubMed] [Google Scholar]
  6. Hanne L. F., Finkelstein R. A. Characterization and distribution of the hemagglutinins produced by Vibrio cholerae. Infect Immun. 1982 Apr;36(1):209–214. doi: 10.1128/iai.36.1.209-214.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmquist B. Characterization of the "microprotease" from Bacillus cereus. A zinc neutral endoprotease. Biochemistry. 1977 Oct 18;16(21):4591–4594. doi: 10.1021/bi00640a009. [DOI] [PubMed] [Google Scholar]
  8. Kessler E., Israel M., Landshman N., Chechick A., Blumberg S. In vitro inhibition of Pseudomonas aeruginosa elastase by metal-chelating peptide derivatives. Infect Immun. 1982 Nov;38(2):716–723. doi: 10.1128/iai.38.2.716-723.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mekalanos J. J., Collier R. J., Romig W. R. Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. J Biol Chem. 1979 Jul 10;254(13):5855–5861. [PubMed] [Google Scholar]
  10. Morihara K. Comparative specificity of microbial proteinases. Adv Enzymol Relat Areas Mol Biol. 1974;41(0):179–243. doi: 10.1002/9780470122860.ch5. [DOI] [PubMed] [Google Scholar]
  11. Mosher D. F. Fibronectin. Prog Hemost Thromb. 1980;5:111–151. [PubMed] [Google Scholar]
  12. Nishino N., Powers J. C. Design of potent reversible inhibitors for thermolysin. Peptides containing zinc coordinating ligands and their use in affinity chromatography. Biochemistry. 1979 Oct 2;18(20):4340–4347. doi: 10.1021/bi00587a012. [DOI] [PubMed] [Google Scholar]
  13. Schneider D. R., Parker C. D. Isolation and characterization of protease-deficient mutants of vibrio cholerae. J Infect Dis. 1978 Aug;138(2):143–151. doi: 10.1093/infdis/138.2.143. [DOI] [PubMed] [Google Scholar]
  14. Schneider D. R., Parker C. D. Purification and characterization of the mucinase of Vibrio cholerae. J Infect Dis. 1982 Apr;145(4):474–482. doi: 10.1093/infdis/145.4.474. [DOI] [PubMed] [Google Scholar]
  15. Woods D. E., Straus D. C., Johanson W. G., Jr, Bass J. A. Role of fibronectin in the prevention of adherence of Pseudomonas aeruginosa to buccal cells. J Infect Dis. 1981 Jun;143(6):784–790. doi: 10.1093/infdis/143.6.784. [DOI] [PubMed] [Google Scholar]
  16. Young D. B., Broadbent D. A. Biochemical characterization of extracellular proteases from Vibrio cholerae. Infect Immun. 1982 Sep;37(3):875–883. doi: 10.1128/iai.37.3.875-883.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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