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. 2009 Feb 17;10:5. doi: 10.1186/1471-2156-10-5

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Copyright © 2009 Garritano et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Simultaneous genotyping of two or more SNPs within the same amplicon using the classic HRM approach. There are two SNPs in this amplicon: rs17880560 and rs1614984. In grey, samples homozygous for both SNPs; in red, samples homozygous for rs17880560 (delCACGGC/delCACGGC) and heterozygous for rs1614984 (C/T). In blue, samples homozygous for rs1614984 (C/C) and heterozygous for rs17880560 (insCACGGC/delCACGGC). In green, samples heterozygous for both SNPs.