Table 2.
Summary of different approaches utilized for each genetic situations.
| Genetic situation | HRM solutions |
| No common SNP in the amplicon | Direct HRM analysis |
| Only one common SNP in the amplicon | Sometimes it is possible to distinguish directly the three groups (Figure 1) Otherwise, spike-in or unlabelled probe is needed (Figure 2) |
| Two SNPs in the amplicon, one is common the other is rare | Spike-in when possible (Figure 3); otherwise, use unlabeled probe for the common SNP, and perform mutation scanning for the rare one (Figure 4) |
| Two or more common SNPs in the amplicon | Use an unlabeled probe for each SNP (Figure 5) |
| Two SNPs in two different amplicons | When Tm of 2 amplicons is different, it is possible to perform multiplex PCR (Figure 6) |