Table 1.
Regulation of selected inflammation-related genes in LPS-activated moDCs, in absence or presence of miR-155
| Affymetrix ID | Gene symbol | Description | Fold change miR-155 (−) moDCs | Fold change miR-155 (+) moDCs |
||
|---|---|---|---|---|---|---|
| 4 h vs 0 h | 16 h vs 4 h | P | ||||
| Cytokines | ||||||
| 205067_at | IL1B | interleukin 1, beta | 2.2* | 174.0 | −3.4 | 3.25 × 10−06 |
| 39402_at | 2.2* | 150.0 | −3.2 | 5.19 × 10−06 | ||
| 210118_s_at | IL1A | interleukin 1, alpha | 1.7 | 287.1 | −30.8 | 1.62 × 10−04 |
| 205207_at | IL6 | interleukin 6 | 1.6 | 239.1 | −7.3 | 5.63 × 10−06 |
| 220054_at | IL23A | interleukin 23, alpha subunit p19 | 2.3* | 20.0 | −8.6 | 7.78 × 10−04 |
| 207113_s_at | TNF | tumor necrosis factor alpha | 1.7 | 53.8 | −14.1 | 5.54 × 10−05x |
| Chemokines | ||||||
| 205114_s_at | CCL3 (MIP1A) | chemokine (C-C motif) ligand 3 | 1.8 | 37.4 | −3.6 | 1.27 × 10−05 |
| 204103_at | CCL4 (MIP1B) | chemokine (C-C motif) ligand 4 | 2.2* | 35.1 | −3.2 | 1.06 × 10−05 |
| 205476_at | CCL20 (MIP3A) | chemokine (C-C motif) ligand 20 | 2.0 | 386.0 | −5.0 | 1.94 × 10−05 |
| 204470_at | CXCL1 (GROA) | chemokine (C-X-C motif) ligand 1 | 2.3* | 237.7 | −5.4 | 2.43 × 10−05 |
| 209774_x_at | CXCL2 (MIP2A) | chemokine (C-X-C motif) ligand 2 | 3.2 | 103.9 | −5.2 | 2.10 × 10−04 |
| 207850_at | CXCL3 (MIP2B) | chemokine (C-X-C motif) ligand 3 | 2.9* | 108.7 | −5.1 | 2.73 × 10−04 |
| Apoptosis | ||||||
| 211368_s_at | CASP1 | caspase 1 (IL-1 beta-converting enzyme) | 2.3* | 3.8 | −2.5 | 2.58 × 10−02 |
| 211366_x_at | 2.2* | 3.3 | −2.6 | 2.51 × 10−02 | ||
| 209970_x_at | 1.8* | 3.1 | −2.3 | 2.89 × 10−02 | ||
| Ematopoiesis | ||||||
| 222670_s_at | MAFB | v-maf musculoaponeurotic fibrosarcoma oncogene homolog B | 2.6* | −2.1 | −2.0 | 3.51 × 10−02 |
The fold regulation of selected inflammation-related genes affected by miR-155 knockdown has been derived from Table S1 (miR-155 (-) moDCs) and compared with the fold regulation obtained in nontransfected LPS-activated moDCs (miR-155 (+) moDCs) in their early (from 0 h to 4 h after LPS) and late (from 4 h to 16 h after LPS) phase of maturation. All the indicated fold changes are derived from Affymetrix microarray experiments and, if presents, multiple probe sets are indicated. The fold changes of miR-155 (-) moDCs derive from 1 single microarray experiment, and the most relevant genes have been confirmed by qPCR (see Figure 3). The fold changes of miR-155 (+) moDCs are a mean of gene expression values derived from 4 independent microarray experiments, each experiment including a different blood donors. For each experiment, samples have been collected from untreated moDCs (0 h) and at 2 different time points after LPS-stimulation (4 h and 16 h). The expression values were first normalized per gene. The 4 h values were then normalized to 0 h (4 h vs 0 h) and the 16 h values to 4 h (16 h vs 4 h). The p-values have been calculated with the software GeneSpring GX 9.0, applying a repeated measures ANOVA test combined to a asymptotic p-value computation, without any correction. IL-1A and IL-1B are indicated in bold.
*Fold change confirmed by qPCR.