TABLE 1.
Fluorescence lifetimes τ (τ1 omitted) of C6-NBD-PC in DOPC/SSM/Chol GUVs at 25 °C The concentration of C6-NBD-PC corresponded to 1 mol% of total lipids. For estimation and explanation of τ2(ld) and τ2(lo), see Material and Methods and Results. Data are presented as average ± S.E. (n = 8). The presence of lipid domains visible by fluorescence microscopy is indicated in the right column.
| DOPC/SSM/Chol | τ2(ld) | τ2(lo) | Visible domains |
|---|---|---|---|
| mol/mol/mol | ns | ns | |
| 10/0/0 | 7.1 ± 0.1 | No | |
| 8/1/1 | 7.6 ± 0.1 | No | |
| 4/4/2 | 7.3 ± 0.1 | Noa | |
| 1/1/1 | 7.0 ± 0.3 | 12.1 ± 0.4 | Yes |
| 3/3/4 | 7.1 ± 0.4 | 11.6 ± 0.1 | Yes |
| 2/4/4 | 7.4 ± 0.2 | 11.4 ± 0.2 | Yes |
| 1/1/8 | 12.4 ± 0.2 | No |
a
Typically, GUVs with a lipid composition of 4/4/2 did not show domain formation. Occasionally, small domains detectable by microscopy were observed