TABLE 2.
Fluorescence lifetimes τ (τ1 omitted) and respective amplitudes α of C6-NBD-PC in POPC/PSM/Chol GUVs at 25 °C The concentration of C6-NBD-PC corresponded to 1 mol% of total lipids. For estimation and explanation of τ and α, see “Experimental Procedures” and “Results.” Amplitudes are given as a percentage of total amplitude (α1 (not shown), α2(ld), and α2(lo)). Data are presented as average ± S.E. (n = 8). Note, lipid domains were not visible by fluorescence microscopy (see “Results”).
| POPC/PSM/Chol | τ2(ld) | α(ld) | τ2(lo) | α(lo) |
|---|---|---|---|---|
| mol/mol/mol | ns | % | ns | % |
| 7.5/2/0.5 | 8.0 ± 0.1 | 83 ± 1 | ||
| 7/2/1a | 8.4 ± 0.1 | 85 ± 1 | ||
| 6/2/2a | 7.8 ± 0.1 | 86 ± 1 | ||
| 5/2/3 | 7.9 ± 0.4 | 37 ± 2 | 10.9 ± 0.1 | 52 ± 3 |
| 4/2/4 | 7.5 ± 0.2 | 25 ± 3 | 11.6 ± 0.1 | 69 ± 2 |
| 2/2/6 | 7.0 ± 0.4 | 21 ± 2 | 11.4 ± 0.1 | 73 ± 2 |
a
Typically, GUVs with a lipid composition of 7/2/1 and 6/2/2 were fitted biexponentially. In some cases a triexponential fit could be applied (not shown)