Fig. 8. Expression of Multiple cytokines are affected by the loss of PLZF expression.
Supernatants generated and collected as for Fig. 7 were analyzed for TNF, IL-13, GMCSF, IL-3, and IL-10 by BD Biosciences Cytokine Bead Arrays. Supernatants were also tested for IL-5, IL-9 and IL-12p70, but these cytokines were not detected under these conditions. Five independent experiments were done. Triplicates from each experiment were averaged and considered as a single data point. Means and standard deviations for the five experiments are shown. The value for the PLZF deficient iNKTs (KO iNKT) was compared to the other three cell types. P values are marked as * <0.05; ** <0.005. P values were derived using a two-tailed, unparied Mann-Whitney U-test. Choices of statistical tests are explained in the methods section.