TABLE 4.
Effect of Aβ and syn-10H scFv on toxicity induced by aggregated Aβ on differentiated and undifferentiated human neuroblastoma cell line (SH-SY5Y)
Aβ (20 μm) was incubated in Buffer A for 5 days at 37 °C without shaking. Samples were preincubated with or without scFv for 2 h at room temperature and then assayed for cytotoxicity. Cell viability was measured using LDH toxicity assay after an additional 2-day incubation of cells and aggregate samples. The data are expressed as percentage of control values from three different experiments repeated in triplicate. Statistical significance was established as p < 0.001 using one-way analysis of variance followed by Bonferroni's post test.
| % LDH release differentiated cell | % LDH release undifferentiated cell | |
|---|---|---|
| Control | 100 ± 3 | 100 ± 4 |
| Aβ alone (2 μm) | 124 ± 4a,b | 104 ± 1 |
| Aβ (2 μm) + syn-10H scFv (1 μm) | 121 ± 5 | 101 ± 1 |
α-Synuclein alone compared with control for both differentiated and undifferentiated cells.
α-Synuclein toxicity compared between differentiated and undifferentiated cells with the same treatment.