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GFP immunofluorescence (green) of the hearts of mice exposed to starvation for various intervals. Cardiomyocytes are stained red with rhodamine-phalloidin and the nuclei are stained blue with Hoechst 33342. Graph showing density (dots per cardiac tissue area) of GFP puncta. *P < 0.05 compared with the value at day 0 (one-way analysis of variance). Scale bar = 10 μm.
