Figure 5.
Histological and immunohistochemical preparations of cardiac tissue from mice normally fed or starved for 3 days with or without BafA1. A: Transverse section of the hearts stained with Masson’s trichrome. B: GFP immunofluorescence (green dots) with nuclear stain by Hoechst 33342 (blue). C: Cathepsin D immunohistochemistry (brown dots). D: Ubiquitin immunohistochemistry (brown dots). E: Double immunofluorescence for TUNEL (green) and myoglobin (red). F: Graphs show the heart-to-body weight ratio, cardiomyocyte size, density of GFP puncta, and %TUNEL-positive noncardiomyocytes in each group. P < 0.05 compared with the fed groups (*) or with the starved group without BafA1 treatment (#) (one-way analysis of variance). Scale bars: 1 mm (A); 10 μm (B–E).