TABLE 1.
PCR primers used for identification of Treponema species in primary endodontic infections
| Target | Primer sequences (5′-3′)a | Positions (bp) | Amplicon length (bp) | Reference |
|---|---|---|---|---|
| Spirochete specific | CAC ATT GGG ACT GAG ATA C | 312-1138 | 827 | 23 |
| TAC CTG TTA GTA ACY GGC AGT AG | ||||
| T. denticola | TAA TAC CGA ATG TGC TCA TTT ACA T | 193-508 | 316 | 3 |
| TCA AAG AAG CAT TCC CTC TTC TTC TTA | ||||
| T. maltophilum | AGA GTT TGA TYM TGG CTC AGb | 8-443 | 436 | 54 |
| CCT ATT GTG CTT ATT CAT CAG GC | ||||
| T. socranskii | GAT CAC TGT ATA CGG AAG GTA GAC A | 148-435 | 288 | 54 |
| TAC ACT TAT TCC TCG GAC AG | ||||
| Universal 16S rRNA gene | AGA GTT TGA TYM TGG CTC AG | 8-1541 | 1,534 | 9, 24 |
| GAA GGA GGT GWT CCA RCC GCA |
a
Y = T or C; M = A or C; W = A or T; R = G or A.
b
Forward universal primer.