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Human-to-mouse reversal mutation at residues L151 restores the ability of hMR1 to activate mouse MAIT cells. The mutation of Leu-151 in hMR1 to Gln of mMR1 rescued the activation of mouse MAIT cells by hMR1 and was blocked by anti-MR1 antibody 26.5. Irrelevant isotype-matched antibody 34–2-12 was used as an isotype control (IsoCtl).
