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. Author manuscript; available in PMC: 2010 Mar 6.
Published in final edited form as: Cell. 2009 Feb 26;136(5):939–951. doi: 10.1016/j.cell.2009.01.025

Figure 3.

Figure 3

PPTR-1 co-localizes with AKT-1. akt-1∷gfp;pptr-1∷mC-flag, akt-2∷gfp;pptr-1∷mC-flag and sgk-1∷gfp;pptr-1∷mC-flag transgenic worms were mounted and visualized by fluorescence microscopy using Rhodamine (mCherry) and FITC (GFP) filters. PPTR-1 expression is observed mainly in the pharynx, vulva and spermatheca (A-C, mCherry).

A) Expression of PPTR-1∷mC-FLAG (mCherry) and AKT-1∷GFP (GFP) in a akt-1∷gfp; pptr-1∷mC-flag strain. Expression of PPTR-1∷mC-FLAG overlaps with AKT-1∷GFP (Merge).

B) PPTR-1∷mC-FLAG and AKT-2∷GFP colocalize in some tissues in a akt-2∷gfp; pptr-1∷mC-flag strain (Merge).

C) SGK-1∷GFP and PPTR-1∷mC-FLAG do not colocalize in sgk-1∷gfp;pptr-1∷mC-flag transgenic worms (Merge).

Arrows indicate the following tissues: p-pharynx, v-vulva, s-spermatheca, i-intestine