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. 2009 Jul 29;4(7):e6413. doi: 10.1371/journal.pone.0006413

Figure 2. GSH depletion in 3T3 fibroblasts. The total cellular GSH concentration was assessed.

Figure 2

The cells were plated as described previously (4) and after attaching 100 µM DEM, or 10 mM BSO, or 100 µM DEM+1 mM GSHe were added. The total cellular concentration of GSH was determined spectrophotometrically as described in “Materials and Methods”. The results are presented as mean±SD of 5–12 different experiments.