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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Cell. 2008 May 16;133(4):704–715. doi: 10.1016/j.cell.2008.03.027

Figure 2.

Figure 2

CD44high/CD24low cells exhibit many properties of stem cells. (A) CD44high/CD24low cells and CD44low/CD24high cells were separated by FACS. (B) Phase-contrast images of mammospheres seeded by CD44high/CD24low (top) and CD44low/CD24high (bottom) cells (C) Quantification of mammospheres formed by cells from the sorted CD44high/CD24low and CD44low/CD24high populations. The data are reported as mean +/− SEM (P<0.001). (D) Images of mammospheres immunostained with antibodies against basal cytokeratin 14 (K14, red) and luminal cytokeratin 8 (K8, green, top panel) or cytokeratin 18 (K18, green, bottom panel). In the merged images (right), the arrows indicate K8/K14 or K18/K14 double-positive cells (yellow). (E) Images of single-cell clones derived from CD44high/CD24low cells (left) or CD44low/CD24high cells (right) stained using antibodies against K14 (red) and K18 (green). The arrows indicate K14/K18 double-positive cells (yellow).