Figure 2.
Soluble factors from SUM102 breast cancer cells stimulate the expression of inflammatory and tumor-promoting genes in normal human mammary fibroblasts (HMFs). HMF cultures were treated for 24h with HMF conditioned media (CM) (control) or 102CM. A. Real-time RT-PCR quantification of HMF mRNA for genes associated with inflammation and CAFs, including MMP-1, MMP-3, IL-8, SDF-1. B. Real-time RT-PCR quantification of HMF mRNA for CXCR4, which is commonly linked to inflammatory, activated fibroblasts, but has not previously associated with CAFs. C. Real-time RT-PCR quantification of MMP-1 and CXCR4 mRNA in HMFs exposed to factors from MDA-MB 231 breast cancer cells (231CM) or LM2 cells (LM2CM). Values were normalized to HPRT and calculated using the 2−(ΔΔC(t)) method, and are presented as fold change from control conditions; bars, SD. *, P<0.05; **, P<0.01; ***P<0.005. Results are representative of five independent experiments.