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. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: J Chromatogr A. 2005 Jul 1;1080(1):68–75. doi: 10.1016/j.chroma.2005.03.035

Table 2.

Effect of ion-pairing reagent hydrophobicity and concentration on retention times of +1, +3 and +5 peptides

Peptide^a	Retention time (min)^b
	10 mM				20 mM				30 mM
	H₃PO₄	TFA	PFPA	HFBA	H₃PO₄	TFA	PFPA	HFBA	H₃PO₄	TFA	PFPA	HFBA
1a	22.21	24.73	27.99	31.74	22.30	25.47	28.72	32.42	22.34	25.76	29.06	32.66
1b	24.81	27.07	30.16	33.73	24.83	27.83	30.87	34.35	24.79	27.95	31.19	34.57
1c	26.98	29.30	32.17	35.63	27.02	29.95	32.88	36.23	27.03	30.16	33.20	36.46
1d	30.61	32.98	35.60	39.01	30.63	33.48	36.26	39.62	30.64	33.80	36.68	39.85
3a	16.93	24.23	33.98	44.31	17.50	26.72	36.26	46.41	17.52	27.63	37.52	47.32
3b	18.81	26.07	35.60	45.47	19.25	28.56	37.90	47.59	19.29	29.55	39.02	48.55
3c	20.72	28.22	37.21	46.75	21.17	30.67	39.55	48.87	21.30	31.58	40.68	49.85
3d	23.42	30.80	39.44	48.67	23.64	33.11	41.75	50.79	23.76	34.08	42.89	51.81
5a	10.67	22.45	39.44	54.69	11.24	26.26	42.64	57.57	10.66	27.95	44.36	58.96
5e	14.87	27.07	42.64	56.62	15.48	30.67	45.91	59.51	15.42	32.54	47.50	60.87
5h	18.21	30.80	45.65	58.61	18.85	34.98	48.79	61.47	19.29	36.44	50.32	62.85
5j	22.82	36.38	49.46	61.44	23.64	39.80	52.61	64.43	23.76	41.26	54.20	65.94

^a

Peptide sequences shown in Table 1.

^b

RP-HPLC conditions, see Section 2.2.