Table 5.
Amplicons used in the current study
| Amplicon | Size (bp) | Primers | Thermal Profile |
|---|---|---|---|
| Amplicon 1 | 165 or 167 bp | 5′-ttcaaggagttcctgctaca-3′ 5′-cattctccattggggatat-3′ |
95°C/10 min, (94°C 20 sec, 56°C/1 min 72°C/45 sec)×35, 72°C/10 min, and then 4°C |
| Amplicon 2 | 155 bp | 5′-tgtcaccttcacagtgacca-3′ 5′-agacactggggcgctta-3′ |
95°C/10 min, (94°C 20 sec, 56°C/1 min 72°C/45 sec)×35, 72°C/10 min, and then 4°C |
| Amplicon 3 | 117 bp | 5′-agtaatagtgtaatactgtaactttaaaatgtgttacttgt-3′ 5′-agccgggatggtctcgat-3′ |
95°C/1.5 min, (94°C/15 sec, 58°/30 sec, 72°/30 sec)×40, 95×/1 min, 60°/1 min |
| β2 microglobulin | 82 bp | 5′-tgggtttcatccatccgacatt-3′ 5′-agacaagtctgaatgctccactttt-3′ |
95°C/1.5 min, (94°C/15 sec, 58°/30 sec, 72°/30 sec)×40, 95°/1 min, 60°/1 min |