. 2009 Aug 3;77(10):4654–4667. doi: 10.1128/IAI.01495-08

TABLE 2.

Primers used in this study

Primer	Locus and gene^a	Sequence (5′ to 3′)^b	Application
0485COMF1	Rv0485	GGTGTCTAGAGTCGTCATCGGGACAACTC	Amplification and cloning of Rv0485 for complementation, incorporates XbaI site at 5′ end of PCR product
0485COMR2	Rv0485	CGTCGAATTCTCAGTGGTGGTGGTGGTGGTGCGCGAGCGCCTCCGGGGC	Amplification and cloning of Rv0485 for complementation, incorporates EcoRI site and codes for His₆ tag at 3′ end of product
0485COMF2	Rv0485	AAGAGAATTCGTGTACTCGACTAACCGCAC	Screening putative complemented clones
0485COMR1	Rv0485	CGTCGAATTCTCACGCGAGCGCCTCCGGGGC	Screening putative complemented clones
pCV129FOR		ATTTGAACGTTAATTAACAACGGCCCGGAGGGTGG	Amplification of 910-bp product from pCV129, introducing PacI site alongside existing AciI site at 5′ end
pCV129REV		GCCTCTTCGCTATTACGCCAGC	Amplification of 910-bp product from pCV129
1195UFOR	Rv1195 and pe13	TCAGTTAATTAATTCGCGGCGAAGG	Amplification of region upstream of Rv1195, incorporates PacI site at 5′ end of product
1195UREV	Rv1195 and pe13	GAAAGCCATGGGCACTCCATCCTC	Amplification of region upstream of Rv1195, incorporates NcoI site at 3′ end of product
1195RTFP1	Rv1195 and pe13	GTGTCTTTCGTGATGGCATACCCA	qRT-PCR
1195RTRP1	Rv1195 and pe13	TCACGGACTGATACATCGCCGCAT	qRT-PCR
1196RTFP1	Rv1196 and ppe18	GAGATGACCAGCGCGGGTGG	qRT-PCR
1196RTRP1	Rv1196 and ppe18	GTTGCTGATCGGCGACCGATGC	qRT-PCR
0485RTFP1	Rv0485	TGTACTCGACTAACCGCACCTCAC	qRT-PCR^c^,^d
0485RTRP1	Rv0485	ACGTAGATCCAGCAATTACGTCCC	qRT-PCR^c^,^d
0485RTFP2	Rv0485	GCTGCTGCGTGACTTACTCAATCC	qRT-PCR^c^,^e
0485RTRP2	Rv0485	AAGGCACCCAATGGATCGGCATAG	qRT-PCR^c^,^e
2391RTFP1	Rv2391	AACGCCAACGAAGAGCTGAAGAAG	qRT-PCR^c
2391RTRP1	Rv2391	AAGTATTTGGCCTCGAGCTTGTCG	qRT-PCR^c
2392RTFP1	Rv2392	ACGTAGTTGCTTCCAACATGGCTG	qRT-PCR^c
2392RTRP1	Rv2392	AACAAGTCCTTGCCCAGCAGTTC	qRT-PCR^c
2393RTFP1	Rv2393	TTCTGTGAGCTCAACTCGCCCAAC	qRT-PCR^c
2393RTFP1	Rv2393	AATGTCCTCACCCAGCACACTG	qRT-PCR^c
2393RTRP1	Rv2394	AACACTTGTGGCGATGCTGGATTG	qRT-PCR^c
2394RTFP1	Rv2394	CCACTGGATTGCTCGGCAAGATTAAC	qRT-PCR^c
2394RTRP1	Rv2625	TTGGTGATCCTGTGGTTGTTCACC	qRT-PCR^f
2625RTFP1	Rv2625	ACAACGTCACGCTCTCAACGGATAC	qRT-PCR^f
2625RTRP1	Rv2626	ACATTGTGATCAAAGGCCTGGCTG	qRT-PCR^f
2625RTRP1	Rv2626	ATGTCGGCTTCGGTGACGATTC	qRT-PCR^f
3132RTFP1	Rv3132	TCCATTGTTGGGCGAACGATTCC	qRT-PCR^f
3132RTRP1	Rv3132	TTGACTCAGCACAACAACGACACC	qRT-PCR^f

ppe19 and ppe60 are highly similar to ppe18.

Restriction sites are underlined.

Upregulated in Rv0485::Tn versus the wild type according to microarray analysis.

Primers used to amplify 5′ region of Rv0485.

Primers used to amplify 3′ region of Rv0485.

Downregulated in Rv0485::Tn versus the wild type according to microarray analysis.