FIG. 2.
Global effects of C. rodentium infection, separated by time after infection and the sex of the mice compared. Points from naive (solid symbols) and infected (open symbols) animals are enclosed in ovals. Each litter is represented by a different symbol (circles, litter A; squares, litter B; triangles, litter C). Blue and yellow indicate tissue and luminal content samples, respectively. Axes show the principal coordinate number and the percent of variation explained. (A) Data for males at day 9 p.i., using unweighted UniFrac. The comparison between luminal contents and tissue samples achieved P < 0.0007 on PCA1; the comparison between naive versus infected achieved significance of P < 0.0001 on PCA2. (B) Same as in panel A, but using weighted UniFrac. The comparison between luminal contents and tissue samples achieved P < 0.0001 on PCA1; the comparison between naive and infected mice achieved a significance of P < 0.0001 on PCA2. (C) Data for day 9 females, unweighted UniFrac. The comparison between luminal contents and tissue samples achieved a significance of P < 0.0028 on PCA2; the comparison between naive and infected achieved a significance of P < 0.0112 on PCA1. (D) Data for day 14 males, unweighted UniFrac. The comparison between luminal contents and tissue samples achieved a significance of P < 0.0149 on PCA1; the comparison between naive and infected achieved a significance of P < 0.0001 on PCA1. (E) Data for day 14 females, unweighted UniFrac. The comparison between luminal contents and tissue samples achieved a significance of P < 0.0001 on PCA2; the comparison between naive and infected achieved a significance of P < 0.0001 on PCA1.