| Reagent for PCR | Volume (μl) for each (25 μl total) | Final concentration (with template) |
|---|---|---|
| Taq DNA polymerase (added last) | 0.25 | 50 U ml−1 |
| 10× buffer | 2.5 | 1× |
| dNTPs (10 mM each, combined) | 0.5 | 200 μM |
| 5′ AgeI—VH or VK primer | 0.5 | 1.2 μM |
| 3′ SalI-JH or 3′ BsiWI-JK primer | 0.5 | 1.2 μM |
| dH2O (nuclease free) | 19.75 | — |
| Template | 1.0 | — |
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