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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Biochemistry. 2009 Jan 20;48(2):399–414. doi: 10.1021/bi8017043

FIGURE 2.

FIGURE 2

A comparison of observed and designed interface sequences. Highlighted are the conserved residues. Hot-spot residues are shown in bold red. A residue is considered hot-spot if the binding free energy change is greater than 2.5 kcal · mol−1 upon mutation to alanine as reported for barnase–barstar (43), for BPTI (44), and for lysozyme–antibody D1.3 (47), respectively. The hot-spot residues of trypsin are not marked since the mutational information is not available.