Table 1. Genes targeted by at least 2 depleted shRNAs (on average >10-fold) in vivo.
| Intended shRNA target | Protein name | Putative role in motility |
|---|---|---|
| cdkn1b | Cyclin-dependent kinase inhibitor p27 | A cell cycle independent function of p27 regulates cell adhesion and migration via interaction with RhoA27. |
| crkl* | v-Crk sarcoma virus CT10 oncogene homolog | An adaptor protein reported to be involved in the activation of Rac22 |
| cyr61 | Cysteine-rich angiogenic inducer 61 | A secreted protein that mediates focal adhesions and is involved in cell attachment and migration 28. |
| ddx11 | RAS-related C3 botulinum substrate 2 | |
| il-6* | Interleukin 6 | |
| map2k3 | Map kinase kinase 3 | Operates upstream of p38 MAPk to regulate cell adhesion29. |
| nek4 | NIMA (never in mitosis gene a)-related kinase 4 | |
| opn5 | Neuropsin | A secreted protein that cleaves fibronectin, inhibiting alpha5 integrin-mediated adhesion30. |
| rac2* | RAS-related C3 botulinum substrate 2 | A hematopoetic-specific Rho GTPase important for the formation of lamellipodia during cell migration20. |
| twf1*1 | Twinfilin, actin-binding protein, homolog 1 (Drosophila) | An actin monomer-binding and actin filament capping protein23. |
| yes1 | v-Yes-1 Yamaguchi sarcoma viral oncogene homolog 1 | Signals downstream of CD95 to promote cell invasion in vivo31. |
*
Subject to further validation
1
Targeted by 3 shRNAs depleted by an average of >10-fold