. Author manuscript; available in PMC: 2010 Jun 1.

Published in final edited form as: Neurobiol Dis. 2009 Mar 28;34(3):487–500. doi: 10.1016/j.nbd.2009.03.004

Table 3.

Identified DA-Conjugated Proteins from DA-Exposed SH-SY5Y Cells

MS Data:

Protein Spot^a	Protein Identification	Accession Number	Theoretical Protein MW	Theoretical Protein pI	Protein Score^b	Protein Score C.I.%^b	Peptide Count
2a. Actin ^*	ACTB protein [Homo sapiens]	gi\|15277503	40194	5.55	108	100	15
2b. Actin ^*	ACTB protein [Homo sapiens]	gi\|15277503	40194	5.55	146	100	16
2c. Actin ^*	actin, gamma 1 propeptide [Homo sapiens]	gi\|4501887	41662	5.31	138	100	17
4. Cloride intracellular channel^*	chloride intracellular channel 1 [Homo sapiens]	gi\|14251209	26792	5.09	119	100	13
7a. ER-60	protein disulfide isomerase family A, member 3, isoformCRA_a [Homo sapiens]	gi\|119597640	54454	6.78	249	100	28
1. protein disulfide isomerase related protein 5 ^*	protein disulfide isomerase-related protein 5 [Homosapiens]	gi\|1710248	46170.2	4.95	131	100	8
3. Tropomyosin	hypothetical protein [Homo sapiens]	gi\|57997573	27387	4.71	199	100	22
5. UCH-L1	ubiquitin carboxyl-terminal esterase L1	gi\|21361091	25151	5.33	148	100	15

Combined MS & MS-MS Data:

Protein Spot^a	Protein Identification	Accession Number	Theoretical Protein MW	Theoretical Protein pI	Protein Score^b	Protein Score C.I.%^b	Total Ion Score^b	Total Ion C.I. %^b	Peptide Count
6. GRP75	heat shock 70kDa protein 9B precursor	gi\|24234688	73920	5.87	145	100	94	100	10
7b. ER-60	protein disulfide isomerase [Homo sapiens]	gi\|860986	57043	6.1	393	100	210	100	21
9. DJ-1	(Q99497) Protein DJ-1 (Oncogene DJ1)	Q99497 ^**	19879	6.33	172	100	78	100	10
8. Peroxiredoxin 2	TSA [Homo sapiens]	gi\|1617118	18486	5.19	325	100	236	100	9
10. Nucleoside diphosphate kinase A	NME1-NME2 protein [Homo sapiens]	gi\|66392203	30346	9.06	228	100	147	100	8
11. SOD1	Chain A, A4v Mutant Of Human Sod1	gi\|47169370	16051	5.7	95	99.993	52	99.86	4

= Protein Spots correspond with Figure 5

= Probability-based MOWSE score (Protein Score) Protein Score Confidence Interval (C.I.) and Total Ion Score and Total Ion C.I.% represent the top score and C.I. pairing obtained across all blots from which the protein was confidently identified

= Identified by MS-based peptide mass fingerprinting in only one blot, but corresponding spots were observed in blots from 2 or more separate experiments For all other identities provided, n = 2-3 for MS analyses, n = 1-3 for MS/MS analyses

^**

= Accession Number from SwissProt database. All others are from NCBI database.