Skip to main content
. Author manuscript; available in PMC: 2009 Nov 3.
Published in final edited form as: J Biol Chem. 2007 Aug 6;282(41):29998–30004. doi: 10.1074/jbc.M702876200

FIGURE 1. Profile of AnxA1 protein expression in human PMN.

FIGURE 1

Resting PMN (A) or PMN stimulated with 10 ng/ml TNF-α (B) were processed for whole lysate (WL), cytosolic (CYT), and membrane (MEM) fractions and immunoblotted with anti-AnxA1 or anti-actin antibody (upper and lower panels, respectively). C, conditioned medium from resting or TNF-α-stimulated PMN was collected and immunoblotted for AnxA1. D, intact and activated PMN (upper and lower panels, respectively) were washed consecutively twice (E1, E2) with 1 mm EDTA to elute membrane-bound AnxA1.