TABLE 2.
Comparison of commercially available, microarray-based kits for detection and identification of respiratory virusesa
| Product | Company | Viruses and/or genotypes detected | Amplification platform(s) | Microarray platform | Characteristic(s) | Reference(s) |
|---|---|---|---|---|---|---|
| Infiniti RVP | AutoGenomics, Inc. (Carlsbad, CA) | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV-A, RSV-B, hMPV-A, hMPV-B, RhV-A, RhV-B, EnV, CoV, and Adv | Multiplex PCR and RT-PCR | Infiniti analyzer (solid chip) | The detection step by the Infiniti analyzer is completely automatic | 160 |
| MultiCode-PLx RVP | EraGen Biosciences (Madison, WI) | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV, hMPV, RhV, AdV, and CoV | Multiplex PCR and RT-PCR | Luminex (liquid chip) | Universal beads used in detection employ EraCode sequences | 109, 143 |
| ResPlex II assay | Qiagen (Valencia, CA) | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV-A, RSV-B, hMPV, RhV, EnV, and severe acute respiratory CoV | Multiplex RT-PCR (Tem-PCR) | Luminex (liquid chip) | A unique Tem-PCR allows large numbers of targets included in one reaction without significant loss of sensitivity | 18, 112 |
| NGEN respiratory virus ASR | Nanogen (San Diego, CA) | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, and RSV | Multiplex RT-PCR | NanoChip (solid chip) | Probe labeling, target capture, and detection are accomplished using electronic microarray technology | 112, 185 |
| xTAG RVP | Luminex Molecular Diagnostics (Toronto, Ontario, Canada) | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV-A, RSV-B, hMPV, AdV, EnV, CoV, and RhV | Multiplex PCR and RT-PCR | Luminex (liquid chip) | TSPE is used in combination with universal detection beads | 126, 132, 148 |
a
Abbreviations: Tem, target-enriched multiplex; Flu, influenza virus; PIV, parainfluenza virus; RSV, respiratory syncytial virus; hMPV, human metapneumovirus; RhV, rhinoviruses; EnV, enteroviruses; CoV, coronavirus; RT, reverse transcription.