Table 1.
Apparent molecular weights of various TFAM deletion constructs, obtained from equilibrium sedimentation AUC
| Single-exponential model |
Double-exponential model |
|||||
|---|---|---|---|---|---|---|
| Apparent Mw (kDa) | Fa | Apparent Mw (kDa) of first species | Fa | Apparent Mw (kDa) of second species | Fa | |
| HMG1 (Calculated Mw = 8.63 kDa) | ||||||
| 50.0 µM | 8.84 ± 0.04 | 1.02 | – | – | – | – |
| 200.0 µM | 8.76 ± 0.01 | 1.02 | – | – | – | – |
| HMG2 (Calculated Mw = 8.07 kDa) | ||||||
| 5.0 µM | 8.68 ± 0.05 | 1.08 | – | – | – | – |
| 50.0 µM | 7.41 ± 0.03 | 0.86 | – | – | – | – |
| 200.0 µM | 8.94 ± 0.01 | 1.11 | – | – | – | – |
| 400.0 µM | – | – | 7.47 ± 0.07 | 0.93 | 13.52 ± 0.15 | 1.68 |
| TFAM (Calculated Mw = 23.95 kDa) | ||||||
| 5.0 µM | – | – | 25.29 ± 0.37 | 1.06 | 54.46 ± 0.65 | 2.27 |
| 10.0 µM | – | – | 25.02 ± 0.75 | 1.04 | 43.75 ± 3.29 | 1.83 |
| 50.0 µM | – | – | 24.71 ± 0.14 | 1.03 | 44.49 ± 0.37 | 1.86 |
| 100.0 µM | – | – | 24.78 ± 0.08 | 1.03 | 53.02 ± 0.55 | 2.21 |
| 150.0 µM | – | – | 22.88 ± 0.30 | 0.96 | 42.45 ± 0.88 | 1.77 |
| 189.4 µM | – | – | 23.18 ± 0.11 | 0.97 | 44.71 ± 0.44 | 1.87 |
| TFAM-ΔC (Calculated Mw = 20.69 kDa) | ||||||
| 10.0 µM | 20.76 ± 0.08 | 1.00 | – | – | – | – |
| 50.0 µM | 20.91 ± 0.03 | 1.01 | – | – | – | – |
| 100.0 µM | 21.07 ± 0.03 | 1.02 | – | – | – | – |
aF = Apprent Mw/calculated Mw.
Data were fitted to either a single-exponential model or a double-exponential model.