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Immunohistochemical detection of cyclin D2, MKI67 (Ki67), and CDKN1B (p27) in ovarian sections. Ovarian sections from adult female WT and granulosa cell-specific SF-1 KO mice were prepared and analyzed by immunohistochemistry as described in Materials and Methods using the indicated specific antibodies. The asterisk indicates the position of the oocyte within the same large follicle in serial sections of the SF-1 KO ovary. Bar = 200 μm.
