TABLE 3.
Measurement of plasma pro-inflammatory markers in high-fat diet-fed mice supplemented with or without 4-PBA
| IL-12p70 (pg/ml) | TNFα (pg/ml) | IFNγ (pg/ml) | MCP-1 (pg/ml) | IL-6 (pg/ml) | |
|---|---|---|---|---|---|
| High fat | 9.69 ± 3.23 | 6.41 ± 0.67 | 2.17 ± 0.49 | 61.05 ± 24.66 | 2.44 ± 0.71 |
| High fat + 4-PBA | 9.13 ± 4.58 | 6.53 ± 1.78 | 1.96 ± 0.38 | 40.87 ± 14.83 | 2.72 ± 1.11 |
Plasma was collected from C57BL/6 mice fed a high-fat diet with or without 4-PBA supplementation at 120 days. Using bead flow cytometry, the concentration of each inflammatory marker was measured. IL-12p70 (n ≥ 6), TNFα (n = 7); IFNγ (n = 7), MCP-1 (n ≥ 6), IL-6 (n = 7). No significant differences were found.