Table II.
Specificity of monoclonal antibodies used for immunohistochemistry
| Ab clone | Isotype | Dilution | Antigen (reference) | Described cross-reactivity (reference) |
|---|---|---|---|---|
| Ca3F4 | IgG2a | 1:5 | Lea (Young et al. 1983) | – |
| 7LE | IgG1 | 1:10 | Lea (Daher et al. 1987) | – |
| BG6 (Signet T218) | IgM | 1:50 | Leb (Sakamoto et al. 1986) | Lec (Monteiro et al. 1998) H type 1 (Monteiro et al. 1998; Manimala et al. 2007) Ley (Manimala et al. 2007) |
| 2.25LE | IgG1 | 1:10 000 | Leb (Bara et al. 1986) | Lea (Good et al. 1992; Amano et al. 1997; Manimala et al. 2007) |
| CA19-9 (Santa Cruz) | IgG1 | 1:500 | sLea (Pour et al. 1988) | – |
| SH1 | IgG3 | 1:5 | Lex (Fukushi, Hakomori, et al. 1984) | – |
| AH6 | IgM | 1:10 | Ley (Abe et al. 1983) | – |
| FH6 | IgM | 1:10 | sLex (Fukushi, Nudelman, et al. 1984) | – |
| KM93 (Calbiochem) | IgM | 1:60 | sLex (Sasaki et al. 1993) | – |