FIG. 8.
Laser scanning confocal immunofluorescence detection of Duox on the apical plasma membrane surface of ciliated primary human bronchial epithelial cells. Images show the apical X-Y plane of confluent cells grown on permeable transwells and re-differentiated for 25 days in an air–liquid interface culture system, as described (84). Ciliated cells, stained with anti β-tubulin antibody (red), are the same as those stained positive for Duox (green). 3-D reconstructed images depicting the rotation of the X-Y plane are available in the supplemental online video, showing Duox and β-tubulin accumulation within the apical aspect of this polarized cell layer. (See online supplemental video at www.liebertonline.com). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).