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. 1988 Jan;85(2):382–386. doi: 10.1073/pnas.85.2.382

Cooperative DNA binding of the yeast transcriptional activator GAL4.

E Giniger 1, M Ptashne 1
PMCID: PMC279552  PMID: 3124106

Abstract

The yeast transcriptional regulatory protein GAL4 binds to four sites in the GAL upstream activating sequence and stimulates transcription of the adjacent GAL1 and GAL10 genes. We show here that binding to at least two of these sites is cooperative in vivo. We also measure stimulation of transcription by pairs of GAL4 binding sites and find that the activities of low-affinity binding sites combine synergistically, whereas the activities of high-affinity binding sites combine only additively. We suggest that the synergistic transcriptional stimulatory activity of the naturally occurring GAL4 binding sites is solely a manifestation of cooperative binding of GAL4 protein to DNA and that the activity of a GAL upstream activating sequence is roughly proportional to the number of GAL4 molecules bound.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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