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For a pool-pool hybridization from the Nimblegen platform depicted are the effect and distribution of probe GC content. Top: Showing the effect of GC content on log-ratio. Bottom: Showing the distribution of probe GC content. The median GC content is 0.42 (IQR 0.38 to 0.44), but is noticeably lower for chromosomes 4 and 13, and noticeably higher for chromosomes 19 and 22. Naturally there is a high spatial auotcorrelation of probe GC content along the genome.
