Skip to main content
. Author manuscript; available in PMC: 2010 Jan 11.
Published in final edited form as: Nat Neurosci. 2007 Apr 8;10(5):578–587. doi: 10.1038/nn1893

Figure 4. PSD–95 mRNA polysomal profile is similar in wildtype and FMR1 knockout mice.

Figure 4

(a) Cytoplasmic brain extract was fractioned by centrifugation on a 5–70% sucrose gradient. 10 fractions were collected while 254 nm absorbance was recorded. RNA was extracted from each fraction and visualized on a denaturing agarose gel. rRNA 28S, 18S and 5S/tRNA are shown in each fraction. Radioactive RT–PCR analysis of total RNA in each fraction with primers specific for PSD-95, β-Actin, Arc and L22 RNAs was performed. The efficiency of translation is reported as a graphic profile of Percentage Messenger on Polysomes (PMP) which was calculated, after normalization to L22, by comparing the radioactivity of the first 5 fractions containing active polysomes to radioactivity from the entire 10 fractions. The PSD–95, β-Actin and Arc PMP in each fraction of wildtype or FMR1 knockout gradients was normalized for L22 RNA. (b) Same as in (a) using cytoplasmic extracts from the hippocampus. PMP value of three independent experiments with standard error is reported. *, p<0.05 for knockout versus wildtype by Student’s t test.