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. 2009 Dec 30;48(3):770–778. doi: 10.1128/JCM.01796-09

TABLE 2.

MLST of C. difficile from ELISA-positive stool samples determined both directly using total stool DNA and using isolates cultured from the same stools

Sample no.a ELISA ODb MLST PCR result (direct) Direct STc Culture resulti Culture STd PCR ribotype
23+ +++++ + 1 +++ 1 027
17+ 0.353 + 1 ++ 1 027
2+ 2.251 + 1 + 1 027
8+ +++++ + 1 +++ 1 027
37+ 1.216 + 1 + 1 027
43+ 0.663 + 1 + 1 027
72+ 2.5 + 1 +++ 1 027
75+ 2.5 + 1 ++ 1 027
85+ 0.388 + 1 + 1 027
88+ 5.584 + 1 +++ 1 027
89+ 5.392 + 1 ++ 1 027
94+ 7.098 + 1 +++ 1 027
98+ 2.5 + 1 +++ 1 027
101+ 1.208 + 1 +++ 1 027
107+ 2.5 + 1 +++ 1 027
60+ +++++ + 1 ++ 1 027
53+ 1.8 + 2 + 2 014
58+ 0.261 + 2 ++ 2 020
27+ +++++ + 2 +++ 2 020
11+ 1.614 + 2 + 2 020
12+ 0.968 + 2 +++ 2 020
13+ 7.718 + 2 +++ 2 020
14+ 3.648 + 2 ++ 2 020
31+ 5.648 + 2 + 2 014
41+ 0.843 + 2 + 2 020
44+ 0.29 + 2 +++ 2 020
48+ 1.003 + 2 ++ 2 076
63+ 2.5 + 2 +++ 2 014
66+ 2.5 + 2 +++ 2 020
67+ 2.5 + 2 ++ 2 014
84+ 0.563 + 2 +++ 2 020
1+ +++++ + 2 ++ 2 076
106+ 1.049 + 2 +++ 2 020
52+ +++++ + 3 ++ 3 072
55+ +++++ + 3 ++ 3 001
57+ 0.285 + 3 + 3 072
16+ +++++ + 3 ++ 3 001
64+ 0.434 + 3 +++ 3 072
65+ 2.5 + 4 ++ 4 137
68+ 1.322 + 4 ++ 4 137
69+ 2.294 + 5 +++ 5 023
76+ 2.5 + 5 ++ 5 023
78+ 2.5 + 5 ++ 5 023
95+ 2.5 + 5 +++ 5 023
59+ 4.083 + 6 + 6 005
51+ +++++ + 6 +++ 6 005
26+ 1.155 + 6 + 6 005
70+ 2.5 + 6 ++ 6 005
92+ 1.896 + 6 +++ 6 005
93+ 0.7 + 6 +++ 6 005
3+ 0.679 + 7 + 7 026
32+ 0.499 + 7 + 7 026
74+ 2.5 + 7 +++ 7 026
39+ 0.27 + 8 +++ 8 002
77+ 2.427 + 8 +++ 8 002
96+ 2.282 + 8 +++ 8 002
97+ 2.379 + 8 +++ 8 002
105+ 2.136 + 8 +++ 8 002
73+ 0.499 + 8 +++ 8 002
79+ 1.163 + 9 + 9 081
7+ 0.35 + 9 + 9 081
80+ 2.5 + 10 +++ 10 015
99+ 0.796 + 10 +++ 10 015
15+ 0.202 + 10 + 10 015
82+ 2.5 + 11 ++ 11 078
4+ 2.13 + 11 +++ 11 078
61+ 0.567 + 11 + 11 078
20+ +++++ + 12 + 12 003
103+ 0.657 + 12 +++ 12 225
108+ 2.5 + 13 +++ 13 129
22+ +++++ + 14 +++ 14 014
5+ 1.658 + 14 + 14 014
33+ 1.658 + 14 ++ 14 014
42+ 0.317 + 14 ++ 14 014
46+ 0.385 + 14 + 14 014
62+ 2.5 + 14 +++ 14 014
19+ 2.257 + 14 + 14 014
36+ 1.09 + 14 ++ 14 014
28+ 0.447 + 19 ++ 19 110
6+ +++++ + 22 + 22 023
49+ 0.41 + 37 ++ 37 017
9+ +++++ + 42 +++ 42 106
24+ 0.182 + 42 + 42 174
40+ +++++ + 42 ++ 42 106
56+ 3.296 + 54 ++ 54 012
104+ 0.387 (+)h NDe +++ 1 027
100+ 0.67 + Mixedg + 5 023
71+ 2.322 + Mixedg +++ 11 078
45+ 2.127 + 1 −
47+ 6.186 + 1 −
50+ 0.278 − + 2 220
86+ 0.369 − + 7 026
91+ 3.578 − +++ 8 002
90+ 1.155 − + NDf NDf
102+ 0.885 − + NDf NDf
54+ 0.26 − −
21+ 7.792 − −
25+ 0.67 − −
29+ 3.475 − −
30+ 0.875 − −
18+ 0.158 − −
34+ 1.487 − −
35+ 0.435 − −
38+ 0.270 − −
81+ 0.537 − −
83+ 0.475 − −
87+ 0.391 − −
a

The plus sign indicates an ELISA-positive result.

b

OD, optical density. +++++, value above the scale.

c

MLST was performed directly from stool samples.

d

MLST was performed from cultured isolates.

e

ND, not done due to very low PCR amplicon yields or no amplicon.

f

ND, not done because sample was negative on reculturing from frozen isolate stock.

g

Evidence of more than one genotype present in stool due to mixed peaks in the nucleotide sequence chromatogram.

h

Poor amplicon yield.

i

Crude estimate of viable C. difficile load. +, <100 colonies; ++, 100 to 1,000 colonies; +++, >1,000 colonies.