Fig. 2.
Dose-dependent effects of wortmannin, Akti-1/2, and rapamycin on SREBP-1c and PEPCK mRNA levels in primary rat hepatocytes. (A) Relative amounts of mRNAs for SREBP-1c (Top) and PEPCK (Bottom) in hepatocytes treated with the indicated concentration of the indicated inhibitor in the absence or presence of insulin as described in Fig. 1. (B) Immunoblot analysis of phosphorylated Akt and S6 ribosomal proteins. Whole-cell lysates were subjected to immunoblot analysis with the indicated antibody as described in Fig. 1D. Filters were exposed to film for 2 sec (P-Akt and P-S6) or 15 sec (Akt and S6). Experiments in A and B were done two times with similar results.