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Confirmation of SodC enzymatic activity. Sonicated cell extracts from E. coli overexpressing the S. typhimurium sodC gene (first and third lanes) or carrying the plasmid vector alone (second and fourth lanes) were subjected to SOD activity gel analysis (13). The left arrow designates SodC, and right arrows designate Fe- and Mn-SOD proteins, respectively. Inhibition of SodC activity by 1 mM sodium cyanide is characteristic of Cu,Zn-SODs (14).
