Fig. 1.
Gene targeting and generation of a conditional knockout (floxed) allele of the StARD4 gene. Targeting strategy employed to insert loxP sites on either side of exon 3 of StARD4. First, a targeting vector was constructed by placing a Neo selectable marker flanked by Frt sites with a loxP site in intron 3. Second, the targeted allele was created by introducing a second loxP in intron 2. Third, crossing the targeted allele mouse to the C57BL/6 ACT-FLPe mouse generated the floxed allele which was used for all subsequent crosses. Finally, to generate the null allele, floxed mice were crossed to the C57BL/6 CMV-Cre.