FIGURE 10.
Autophagy is necessary for the rapamycin-mediated decrease in Aβ levels. 7PA2 cells were treated with various concentrations of rapamycin as indicated for 24 h. At the end of the treatment, proteins were extracted and soluble Aβ42 levels were measured by ELISA. Although rapamycin administration significantly decreased Aβ42 levels (p < 0.05), blocking autophagy with 3-methyladenine (3-MA) prevented the rapamycin-induced decrease in Aβ42 levels. All experiments shown were done in triplicate in three independent experiments; thus, we analyzed a total of 9 samples for each cell line, for each specific condition. Data are presented as mean ± S.E.