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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Jul 31;183(5):3109–3117. doi: 10.4049/jimmunol.0900399

Table I.

DNA fragments used in this study

Size (bp) % GC Optimala
CpG
Non-
optimalb
CpG
M.SssI
site (cg)
Alu I site
(agct)
CG50 607 52.0 50 0 50 0
CGSubOp 727 52.8 0 27 27 6
CGneg 629 42.4 0 0 0 -
pCpG-mcs 3070 38.9 0 0 0 -

CpG island
Clone-11
573 58.6 2 42 44 -
CpG island
Clone-12
453 52.5 0 19 19 -
CpG island
Clone-14
597 49.6 1 38 39 -
CpG island
Clone-15
587 56.7 3 34 37 -
CpG island
Clone-23
574 52.6 0 40 40 -
SUMO 618 33.0 0 1 1 -
a

Optimal CpG: purine-purine-unmethylated cytosine–guanine-pyrimidine-pyrimidine

b

Non-optimal CpG: unmethylated cytosine–guanine, with flanking bases different from optimal CpG motif.