Figure 10. Dnmt agonist promotes, and Dnmt antagonist inhibits, the regeneration of injured sensory spinal axons into a peripheral nerve graft.
Spinal axonal regeneration was measured in the SCRM model on the “conditioned” side after i.p. treatment with a Dnmt agonist 3-ABA (15 mg/kg) or antagonist 5-AzaC (0.1 mg/kg) with and without FA at 80 μg/kg. Treatment with the antagonist suppresses the proregenerative effect of FA (A). Conversely, treatment with the agonist at this dose significantly improves spinal regeneration into a nerve graft in vivo. The effect of 3-ABA at 15 mg/kg is equivalent to the 60 μg/kg dose of FA (7) (B). n = 7 (FA); n = 9 (FA and 5-AzaC); n = 11 (vehicle control); n = 9 (3-ABA); n = 7 (FA); n = 9 (FA and 3-ABA). One-way ANOVA with Bonferroni’s correction; mean ± SEM; *P < 0.05.