Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jan;65(1):206–213. doi: 10.1073/pnas.65.1.206

Temperature-Sensitive Mutants of Bacillus subtilis, I. Multiforked Replication and Sequential Transfer of DNA by a Temperature-Sensitive Mutant*

Hiroshi Yoshikawa 1,†
PMCID: PMC286211  PMID: 4984231

Abstract

A temperature-sensitive mutant of Bacillus subtilis 168 was isolated. Its chromosome was found to undergo multiforked replication at normal temperature. The mutant cells then transferred chromosomes to recipient cells sequentially from the origin to the terminus of the chromosome at sublethal temperatures. Sequential transfer of the chromosome facilitated the determination of the relative positions of markers on the chromosome. Linkage between origin (adenine-16) and terminus (methionine) was demonstrated.

Full text

PDF
206

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  2. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  3. Gross J. D., Karamata D., Hempstead P. G. Temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Cold Spring Harb Symp Quant Biol. 1968;33:307–312. doi: 10.1101/sqb.1968.033.01.034. [DOI] [PubMed] [Google Scholar]
  4. Haas M., Yoshikawa H. Defective bacteriophage PBSH in Bacillus subtilis. II. Intracellular development of the induced prophage. J Virol. 1969 Feb;3(2):248–260. doi: 10.1128/jvi.3.2.248-260.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LARK C., LARK K. G. EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964 Oct;10:120–136. doi: 10.1016/s0022-2836(64)80032-2. [DOI] [PubMed] [Google Scholar]
  6. O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
  7. Pakula R. Cellular sites for the competence-provoking factor of streptococci. J Bacteriol. 1967 Jul;94(1):75–79. doi: 10.1128/jb.94.1.75-79.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Spizizen J., Reilly B. E., Evans A. H. Microbial transformation and transfection. Annu Rev Microbiol. 1966;20:371–400. doi: 10.1146/annurev.mi.20.100166.002103. [DOI] [PubMed] [Google Scholar]
  9. Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
  10. Sueoka N., Yoshikawa H. The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics. 1965 Oct;52(4):747–757. doi: 10.1093/genetics/52.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TAKAHASHI I. INCORPORATION OF BACTERIOPHAGE GENOME BY SPORES OF BACILLUS SUBTILIS. J Bacteriol. 1964 Jun;87:1499–1502. doi: 10.1128/jb.87.6.1499-1502.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yoshikawa H. DNA synthesis during germination of Bacillus subtilis spores. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1476–1483. doi: 10.1073/pnas.53.6.1476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yoshikawa H., Haas M. On the regulation of the initiation of DNA replication in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:843–855. doi: 10.1101/sqb.1968.033.01.096. [DOI] [PubMed] [Google Scholar]
  15. Yoshikawa H. Mutations Resulting from the Transformation of BACILLUS SUBTILIS. Genetics. 1966 Nov;54(5):1201–1214. doi: 10.1093/genetics/54.5.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshikawa H. The initiation of DNA replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jul;58(1):312–319. doi: 10.1073/pnas.58.1.312. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES