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. 1989 Jan;86(1):302–306. doi: 10.1073/pnas.86.1.302

Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells.

R G Goodwin 1, S Lupton 1, A Schmierer 1, K J Hjerrild 1, R Jerzy 1, W Clevenger 1, S Gillis 1, D Cosman 1, A E Namen 1
PMCID: PMC286452  PMID: 2643102

Abstract

A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.

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Selected References

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